live-death cell imaging kit  (Thermo Fisher)

Journal: Mediators of Inflammation

Article Title: IL-37 Exerts Anti-Inflammatory Effects in Fetal Membranes of Spontaneous Preterm Birth via the NF- κ B and IL-6/STAT3 Signaling Pathway

doi: 10.1155/2020/1069563

Figure Lengend Snippet: Effects of IL-37 on apoptosis and proliferation in human amniotic epithelial cells. (a) Cell proliferation between the IL-37 siRNA group and the si_NC group was measured by 5-ethynyl-20-deoxyuridine (EdU) incorporation assay. The blue color indicates the nuclei, and the red color represents the EdU-positive nuclei. Scale bar: 200 μ m. (b) Statistical analysis of EdU staining in the result (a). (c) The Live/Death cell kit was used to analyze the number of dead cells between the IL-37 siRNA group and the si_NC group. The blue color indicates the nuclei, and the green color represents the death cells. Scale bar: 200 μ m. (d) Statistical analysis of death cell rate in the result (c). (e) Cell apoptosis between the IL-37 siRNA group and the si_NC group was assessed by the flow cytometer. Representative pictures of flow cytometry for apoptosis rate. (f) Statistical analysis of flow cytometry in the result (e). (g) The apoptosis-related protein biomarkers were measured by western blot in each group. (h) Statistical analysis of western blot in the result (g). (i) The STAT3 signaling was measured by western blot between the si_NC group and the IL-37 siRNA group. (j) Statistical analysis of western blot in the result (i). ∗ p < 0.05 vs. si_NC; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Article Snippet: Two fluorescent probes (Live/Death Cell Imaging Kit, Invitrogen, USA) (1 drop/500 μ l medium) were used in each well and then incubated for 15 minutes prior to photography under fluorescence microscopy.

Techniques: Staining, Flow Cytometry, Western Blot